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Home>News Articles>Highlights of Accomplishments in 2008>FFTC, NIFTS and NTU holds workshop on LAMP method for HLB pathogen detection
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 January 16 2008
Participants of the LAMP workshop with Prof. Hong-Ji Su (rightmost), FFTC Consultant and Professor Emeritus, NTU.

Participants of the LAMP workshop with Prof. Hong-Ji Su (rightmost), FFTC Consultant and Professor Emeritus, NTU.

TAIPEI, TAIWAN ROC - A one-day workshop on "Application of LAMP method for detection of HLB (citrus greening disease) pathogen" was held in September 2007 at the National Taiwan University (NTU) as part of a collaborative research among FFTC, NTU and the National Institute of Fruit Tree Science (NIFTS), Japan. Two Japanese scientists, Dr. M. Okuda of the National Agricultural Research Center for Kyushu Okinawa Region (KONARC) and Dr. S. Miyata of NIFTS were invited to give a lecture and laboratory demonstration on Loop-mediated isothermal amplification (LAMP) for HLB pathogen detection. Under the coordination of Prof. Hong-Ji Su, FFTC Consultant and NTU Professor Emeritus, more than 10 scientists engaged in HLB research all over Taiwan participated in the workshop.

Polymerase chain reaction (PCR) is the most common method used for the detection of HLB pathogen. However, this method requires a thermal cycler and other basic apparatus for molecular biological experiments, as well as trained personnel, which are not always present in many laboratories especially in developing countries in the Asian region.

Loop-mediated isothermal amplification (LAMP), on the other hand, is a newly developed method for DNA amplification in Japan. This method is considered to have several advantages over the PCR, such as: 1) DNA amplification is catalyzed by only one enzyme; 2) Highly efficient amplification (within 30 min using a simple, affordable water bath); 3) Highly specific detection of HLB pathogen by four different kinds of primer; and 4) Large quantity of products can be amplified even in under-equipped laboratories of extension centers and local quarantine offices.